Effect of Adsorption Condition on Thermal Stability of Proteins Adsorbed onto Biomass Charcoal Powder
نویسندگان
چکیده
Authors have found out that bamboo charcoal powder (BCP), which is prepared from bamboo wastes by pyrolysis at low temperatures, is very useful as a carrier for the thermal stabilization of proteins. Hen egg white lysozyme (HEWL) and α-chymotrypsin (CT) were effectively adsorbed onto BCP. The thermal stability of BCP-adsorbed HEWL was strongly dependent upon the adsorption conditions such as solution pH, ionic strength, and temperature. Moreover, writers have revealed the heat-resistant mechanism on the basis of the structure of HEWL. Likewise, the thermal stability of BCP-adsorbed CT could be enhanced by selecting the adsorption condition.
منابع مشابه
Heat-Resistant Properties of α-Chymotrypsin Adsorbed onto Biomass Charcoal Powder
Biomass charcoal powder (BCP) was used as a carrier for immobilization of α-chymotrypsin through adsorption. BCP was derived from plant biomass wastes such as dumped bamboos by oxygen-free pyrolysis at low temperatures and grinding with a jet mill. The activity of adsorbed α-chymotrypsin was strongly dependent upon the kind of BCP. The thermal denaturation curve of adsorbed α-chymotrypsin was s...
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